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human passage 10 20 22rv1  (ATCC)


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    ATCC human passage 10 20 22rv1
    (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) <t>22Rv1</t> and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.
    Human Passage 10 20 22rv1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2870 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+passage+10+20+22rv1/pmc06342284-35-0-5?v=ATCC
    Average 99 stars, based on 2870 article reviews
    human passage 10 20 22rv1 - by Bioz Stars, 2026-08
    99/100 stars

    Images

    1) Product Images from "Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator"

    Article Title: Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator

    Journal: Cell reports

    doi: 10.1016/j.celrep.2018.11.035

    (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) 22Rv1 and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.
    Figure Legend Snippet: (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) 22Rv1 and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Techniques Used: Knockdown, Expressing, Control, Transfection, Quantitative RT-PCR, Over Expression, Infection, shRNA

    (A–D) EZH2 knockdown decreases AR mRNA and protein levels. LNCaP (A), LAPC4 (B), C4–2B (C), and 22RV1 (D) cells were transfected with control or siEZH2s or infected with control shRNA or shEZH2, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR mRNA and protein levels. LNCaP (E) and LAPC4 (F) cells were infected with CMV or an EZH2-expressing adenovirus for 48 hr, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three.
    Figure Legend Snippet: (A–D) EZH2 knockdown decreases AR mRNA and protein levels. LNCaP (A), LAPC4 (B), C4–2B (C), and 22RV1 (D) cells were transfected with control or siEZH2s or infected with control shRNA or shEZH2, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR mRNA and protein levels. LNCaP (E) and LAPC4 (F) cells were infected with CMV or an EZH2-expressing adenovirus for 48 hr, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Techniques Used: Knockdown, Transfection, Control, Infection, shRNA, Quantitative RT-PCR, Western Blot, Over Expression, Expressing

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, Western Blot, Isolation, cDNA Synthesis, Reporter Assay, Imaging, Control, Software



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    human passage 10 20 22rv1  (ATCC)
    99
    ATCC human passage 10 20 22rv1
    (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) <t>22Rv1</t> and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.
    Human Passage 10 20 22rv1, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+passage+10+20+22rv1/pmc06342284-35-0-5?v=ATCC
    Average 99 stars, based on 1 article reviews
    human passage 10 20 22rv1 - by Bioz Stars, 2026-08
    99/100 stars
    		  Buy from Supplier

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    (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) 22Rv1 and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Journal: Cell reports

    Article Title: Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator

    doi: 10.1016/j.celrep.2018.11.035

    Figure Lengend Snippet: (A and B) Androgen-induced genes (A) are enriched for downregulation upon EZH2 knockdown (false discovery rate [FDR] q < 0.001), whereas androgen-repressed genes (B) are enriched for upregulation upon EZH2 knockdown (FDR q < 0.001). GSEA was utilized to examine the expression of androgen (R1881)-induced and -repressed gene sets, obtained from a previous study , in LNCaP cells treated with control (siCtrl) and EZH2 knockdown (siEZH2), as profiled by microarrays. (C and D) EZH2 knockdown inhibits AR-induced genes. LNCaP cells (C) were transfected with siCtrl or two different siEZH2s, and LAPC4 (D) cells were transfected with siCtrl or a representative siEZH2. Cells were then analyzed by qRT-PCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR-induced genes. LNCaP (E) and LAPC4 (F) cells were infected with cytomegalovirus (CMV) control or an EZH2-expressing adenovirus and analyzed by qRTPCR. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (G and H) EZH2 knockdown reduces AR-induced genes in CRPC cells. (G) 22Rv1 and (H) C4–2B cells were infected with control shRNA or shEZH2 or transfected with either siCtrl or two different siEZH2s and then subjected to qRT-PCR analysis. Data were normalized to GAPDH. Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Article Snippet: Human: Passage 10–20 22Rv1 , ATCC , Cat# CRL-2505; RRID:CVCL_1045.

    Techniques: Knockdown, Expressing, Control, Transfection, Quantitative RT-PCR, Over Expression, Infection, shRNA

    (A–D) EZH2 knockdown decreases AR mRNA and protein levels. LNCaP (A), LAPC4 (B), C4–2B (C), and 22RV1 (D) cells were transfected with control or siEZH2s or infected with control shRNA or shEZH2, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR mRNA and protein levels. LNCaP (E) and LAPC4 (F) cells were infected with CMV or an EZH2-expressing adenovirus for 48 hr, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Journal: Cell reports

    Article Title: Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator

    doi: 10.1016/j.celrep.2018.11.035

    Figure Lengend Snippet: (A–D) EZH2 knockdown decreases AR mRNA and protein levels. LNCaP (A), LAPC4 (B), C4–2B (C), and 22RV1 (D) cells were transfected with control or siEZH2s or infected with control shRNA or shEZH2, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three. (E and F) EZH2 overexpression increases AR mRNA and protein levels. LNCaP (E) and LAPC4 (F) cells were infected with CMV or an EZH2-expressing adenovirus for 48 hr, followed by qRT-PCR (left) and western blot analysis (right). Data shown are mean (±SEM) of technical replicates from one representative experiment of three.

    Article Snippet: Human: Passage 10–20 22Rv1 , ATCC , Cat# CRL-2505; RRID:CVCL_1045.

    Techniques: Knockdown, Transfection, Control, Infection, shRNA, Quantitative RT-PCR, Western Blot, Over Expression, Expressing

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Polycomb- and Methylation-Independent Roles of EZH2 as a Transcription Activator

    doi: 10.1016/j.celrep.2018.11.035

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Human: Passage 10–20 22Rv1 , ATCC , Cat# CRL-2505; RRID:CVCL_1045.

    Techniques: Recombinant, Western Blot, Isolation, cDNA Synthesis, Reporter Assay, Imaging, Control, Software